Namibian spitting cobra, Naja nigricincta nigricincta (zebra snake): Oral flora and antibiotic sensitivity, a cross-sectional study
DOI:
https://doi.org/10.7196/SAMJ.2023.v113i7.271Keywords:
Naja nigricincta nigricincta, snake bite, wound infection, oral flora Enterococcus faecalis, Proteus spp, Morganella morganiiAbstract
This was a cross-sectional study with the aim of characterising Naja nigricincta nigricincta’s oral bacterial flora as well as accompanying sensitivities and resistance towards antibiotics. Naja nigricincta nigricincta (zebra snake) is a spitting cobra indigenous to Namibia. Nasopharyngeal and venom swabs for bacterial culture and antibiotic sensitivity were taken from 37 native zebra snakes originating from the Khomas region that were captured for removal and relocation. Enterococcus faecalis, Proteus spp., Morganella morganii and Pseudomonas spp. were the organisms most often cultured. The antibiotic sensitivity profiles of these organisms suggest ciprofloxacin or a third-generation cephalosporin plus gentamicin or piperacillin-tazobactam as prophylactic antibiotics in case of Naja nigricincta nigricincta bites.
References
Buys PJC. Medical management of snakebite in Namibia. Windhoek: Gamsberg Macmillan, 2003.
Saaiman E, Buys P. Spitting cobra (Naja nigricincta nigricincta) bites complicated by rhabdomyolysis, possible intravascular haemolysis and coagulopathy. S Afr Med J 2019;109(10):736. https://doi.
org/10.7196/SAMJ.2019.v109i10.14103
Tilbury CR. Observations on the bite of the Mozambique spitting cobra (Naja mossambica mossambica). S Afr Med J 1982;61(9):308-313. https://hdl.handle.net/10520/AJA20785135_14484 (accessed 15 May 2023).
Wagener M, Naidoo M, Aldous C. Wound infection secondary to snakebite. S Afr Med J 2017;107(4):315-319. https://doi.org/10.7196/SAMJ.2017.v107i4.12084
Warrell DA, Greenwood BDNOLPC. Necrosis, haemorrhage and complement depletion following bites by the spitting cobra. QJM 1976;45(1):1-22. https://doi.org/10.1093/oxfordjournals.qjmed.a067448
Saaiman EL, Buys PJC. Fatal infective necrotising fasciitis: Complication following Naja nigricincta nigricincta bite (western barred spitting cobra/zebra snake). S Afr Med J 2022;112(12):892-896. https:// doi.org/10.7196/SAMJ.2022.v112i12.16689
Griffen M. The Zebra snake antivenom project. Travel News Namibia, 2001. https://www. travelnewsnamibia.com/news/stories/featured-stories/the-zebra-snake-antivenom-project/ (accessed 8 October 2018).
Marais J. A Complete Guide to the Snakes of Southern Africa. 2nd ed. Cape Town: Struik, 2004.
BlaylockRS.TheidentificationandsyndromicmanagementofsnakebiteinSouthAfrica.SAfrFamily
Pract 2005;47(9):46-53. https://doi.org/10.1080/20786204.2005.10873288
Tsai Y-H, Hsu W-H, Huang K-C, Yu P-A, Chen C-L, Kuo LT. Necrotizing fasciitis following
venomous snakebites in a tertiary hospital of southwest Taiwan. Int J Infect Di. 2017;63:30-36. https://
doi.org/10.1016/j.ijid.2017.08.005
Gutiérrez JM, Rucavado A, Chaves F, Díaz C, Escalante T. Experimental pathology of local tissue
damage induced by Bothrops asper snake venom. Toxicon 2009;54(7):958-975. https://doi.
org/10.1016/j.toxicon.2009.01.038
Houcke S, Resiere D, Lontsingoula GR, et al. Characteristics of snakebite-related infection in French Guiana. Toxins 2022;14(2):89. https://doi.org/10.3390/toxins14020089
Saravia-Otten P, Gutiérrez J, Arvidson S, Thelestam M, Flock J-I. Increased infectivity of Staphylococcus aureus in an experimental model of snake venom-induced tissue damage. J Infect Dis 2007;196(5):748- 754. https://doi.org/10.1086/520537
Huang L-W, Wang J-D, Huang J-A, Hu S-Y, Wang L-M, Tsan Y-T. Wound infections secondary to snakebite in central Taiwan. J Venomous Animals Toxins incl Trop Dis 2012;18(3):272-276. https:// doi.org/10.1590/S1678-91992012000300004
Resiere D, Gutierrez JM, Neviere R, Cabie A, Hossein M, Kallel H. Antibiotic therapy for snakebite envenoming. J Venom Anim Toxins incl Trop Dis 2020;26. https://doi.org/10.1590/1678-9199- jvatitd-2019-0098
Sadeghi M, Barazandeh M, Zakariaei Z, et al. Massive cutaneous complications due to snakebite: A case report and literature review. Clin Case Rep 2021;9(5):e04129. https://doi.org/10.1002/ccr3.4129
Garg A, Sujatha S, Garg J, Acharya NS, Parija SC. Wound infections secondary to snakebite. J Infect Dev Ctries 2009;3(3):221-223. https://doi.org/10.3855/jidc.39
Lin JH, Sung WC, Mu HW, Hung DZ. Local cytotoxic effects in cobra envenoming: A pilot study. Toxins 2022;14(2):122. https://doi.org/10.3390/toxins14020122
Chuang P-C, Lin W-H, Chen Y-C, Chien C-C, Chiu I-M, Tsai T-S. Oral bacteria and their antibiotic susceptibilities in Taiwanese venomous snakes. Microorganisms 2022;10(5):951.
Mao Y-C, Chuang H-N, Shih C-H, et al. An investigation of conventional microbial culture for the Naja atra bite wound, and the comparison between culture-based 16S Sanger sequencing and 16S metagenomics of the snake oropharyngeal bacterial microbiota. PLoS Negl Trop Dis 2021;15(4):e0009331. https://doi.org/10.1371/journal.pntd.0009331
Yak R, Lundin A-C, Pin P, Sebastin S. Oral bacterial microflora of free-living reticulated pythons (Python reticulatus) in Singapore. J Herpetol Med Surg 2015;25:40-44. https://doi.org/10.5818/1529- 9651-25.1.40
Blaylock RS. Antibiotic use and infection in snakebite victims. S Afr Med J 1999;89(8):874-876.
Chen CM, Wu KG, Chen CJ, Wang CM. Bacterial infection in association with snakebite: A 10-year experience in a northern Taiwan medical center. J Microb Immunol Infect 2011;44(6):456-460. https://
doi.org/10.1016/j.jmii.2011.04.011
Atkins MCP, Larsen KW. Measuring snakes across the decades. Are tube-restraint measurements compatible with an earlier method? Herpetol Conserv Biol 2020;15(2):350-353.
Chippaux JP, Williams V, White J. Snake venom variability: Methods of study, results and interpretation. Toxicon 1991;29(11):1279-1303. https://doi.org/10.1016/0041-0101(91)90116-9
Spawls S, Branch B. The Dangerous Snakes of Africa. London: Bloomsbury Publishing, 2020.
Samy RP, Stiles BG, Franco OL, Sethi G, Lim LHK. Animal venoms as antimicrobial agents. Biochem
Pharmacol 2017;134:127-138. https://doi.org/10.1016/j.bcp.2017.03.005
Ul-Hasan S, Rodríguez-Román E, Reitzel AM, et al. The emerging field of venom-microbiomics for
exploring venom as a microenvironment, and the corresponding Initiative for Venom Associated Microbes
and Parasites (iVAMP). Toxicon X 2019;4:100016. https://doi.org/10.1016/J.TOXCX.2019.100016
Esmaeilishirazifard E, Usher L, Trim C, et al. Microbial adaptation to venom is common in snakes and
spiders. bioRxiv 2018;348433. https://doi.org/10.1101/348433
Zancolli G, Mahsberg D, Sickel W, Keller A. Reptiles as reservoirs of bacterial infections: Real threat or
methodological bias? Microb Ecol 2015;70(3):579-584.
Smith SN, Colston TJ, Siler CD. Venomous snakes reveal ecological and phylogenetic factors influencing variation in gut and oral microbiomes. Front Microbiol 2021;12:657754. https://doi.org/10.3389/fmicb.2021.657754
Li X, Du Z, Tang Z, Wen Q, Cheng Q, Cui Y. Distribution and drug sensitivity of pathogenic bacteria in diabetic foot ulcer patients with necrotizing fasciitis at a diabetic foot center in China. BMC Infect Dis 2022;22(1):1-10. https://doi.org/10.1186/s12879-022-07382-7
Auffenberg W. The Behavioral Ecology of the Komodo Monitor. Gainesville: University Press of Florida, 1981.
Fry B. Venomous Reptiles and their Toxins: Evolution, Pathophysiology and Biodiscovery. Oxford: Oxford University Press, 2015.
Masoumi B, Eslami G, Alizadeh-Navaei R, Mondal P, Rezai MS. Safety profile of using ciprofloxacin in paediatrics: A systematic review and meta-analysis. J Pediatr Rev 2019;7(3):129-140. http://doi. org/10.32598/jpr.7.3.129
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Esta Saaiman, Christo Buys

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Licensing Information
The SAMJ is published under an Attribution-Non Commercial International Creative Commons Attribution (CC-BY-NC 4.0) License. Under this license, authors agree to make articles available to users, without permission or fees, for any lawful, non-commercial purpose. Users may read, copy, or re-use published content as long as the author and original place of publication are properly cited.
Exceptions to this license model is allowed for UKRI and research funded by organisations requiring that research be published open-access without embargo, under a CC-BY licence. As per the journals archiving policy, authors are permitted to self-archive the author-accepted manuscript (AAM) in a repository.
Publishing Rights
Authors grant the Publisher the exclusive right to publish, display, reproduce and/or distribute the Work in print and electronic format and in any medium known or hereafter developed, including for commercial use. The Author also agrees that the Publisher may retain in print or electronic format more than one copy of the Work for the purpose of preservation, security and back-up.





